威尼斯DG梦幻厅

Reagent Center

Human Pathogen Series

  • SARS-CoV-2 Lyophilized Nucleic Acid Detection Kit MORE
    Product Details:

    This real-time RT-PCR test kit is designed for the qualitative detection of SARS-CoV-2 RNA from human nasopharyngeal swabs, oropharyngeal swabs, sputum, alveolar lavage fluid specimens. This kit is a real-time reverse transcription polymerase chain reaction (rRT -PCR) detection kit, specially targeting ORF1ab gene and N gene of SARS-CoV-2. It also targets endogenous internal control (β-globin gene) to monitor the quality of the specimen collection and extraction process. The lyophilized powder is designed for easy storage and transportation while ensuring the sensitivity and accuracy of the kit.

     

    Components

    SARS-CoV-2 reaction

    mixture (lyophilized)、SARS-CoV-2 positive control (lyophilized)、ultrapure water、paraffin oil 

    Pack Size

    12×8T/Kit; 96T/Kit

    Storage Conditions

    1. Store between -25℃ to 8℃ away from light, valid for 12 months

    2. Lyophilized reaction mixture after reconstitution with water: Store between -25℃ to -15℃. Away from light, valid for 1 month

    Transportation Conditions

    ≤37℃, stable for 3 months.

    Test Samples

    Human nasopharyngeal swabs, oropharyngeal swabs, sputum, alveolar lavage fluid specimens

    Special Instrument

    UltraFast QPCR, and regular Real-Time PCR Systems such as LineGene 9600 Plus, QuantGene 9600, ABI 7500, LightCycler96, TL988, SLAN Real-Time PCR System

    Note: Paraffin oil is required only when this test kit is used in the device of UltraFast QPCR. Do not use paraffin oil when this test kit is used with regular Real-Time PCR Systems.

    Product Features:
    1.Extremely strong thermal stability: resistant to high temperature treatment, unchanged performance

    2.Storage and transportation at room temperature:the lyophilized kit does not need cold chain transportation and does not need to be stored at low temperature before opening to fully release the cold storage space

    3.One step method: ;full component lyophilized, avoid the loss of high viscosity components such as enzymes, more convenient operation

    4.Wide detection range: samples conclude human nasopharyngeal swabs, oropharyngeal swabs, sputum and alveolar lavage fluid specimens

    5.Strong specificity: There was no cross reaction with other kinds of common clinical pathogens, and the common interferences did not affect the kit

    Experimental data:

    The standard SARS-CoV-2 nucleic acid was diluted to 400copies/mL and 250copies/mL with 2ng/ L human genomic DNA product, respectively. Each concentration was repeated 20 times. The results were as follows:

     

    Manual download
  • SARS-CoV-2/ Influenza A Virus/ Influenza B Virus Nucleic Acid Detection Kit (Fluorescence RT-PCR) MORE
    Product Details:

    The SARS-COV-2, influenza A virus and influenza B virus are infectious and harmful, and can cause similar clinical symptoms and complications. This kit adopts fluorescence quantitative RT-PCR method, which can be timely diagnosed in the early stage of virus infection, and can distinguish the virus type. By labeling different fluorescent signals on the designed specific probes, different fluorescence was generated in the reaction system, so that the three viral nucleic acids could be detected by type in the same reaction tube which greatly improves the work efficiency.

    Product Features:
    1. 3 in 1: The SARS-COV-2, influenza A virus and influenza B virus nucleic acid can be tested in one reaction of the kit .It can be used to rapidly triage people with related suspected symptoms.
    2. Strong applicability: suitable for human nasopharyngeal swabs
    3. High sensitivity: Three different batches of reagent were used for testing, and the detection sensitivity could reach 500 copies/mL
    4. High specificity: no cross reaction with meningococcus, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, etc. The presence of blood, mucin, and nasal secretions did not affect the detection of the sample.
    5. High accuracy: ORF1ab and N genes of SARS-COV-2, 5 subtypes of influenza A viruses (A H1N1, A H3N2, A H5N1, A H7N9) and Victoria and Yamagata strains of influenza B viruses can be detected simultaneously. Reference test results: positive coincidence rate is 100%, negative coincidence rate is 100%.
    6. Simple operation: one-step method to complete RT-PCR, The whole procedure can be detected within 80min.
    Experimental data:

    Case 1: Three pseudovirus containing ORF1ab and N genes of SARS-COV-2, influenza A genes, and influenza B genes were premixed and diluted in accordance with a 10-fold gradient. MagaBio plus Virus DNA/RNA Purification Kit III was used for purification.

    Figure 1 SARS-CoV-2, influenza A virus and influenza B virus mixed samples

    The results showed that SARS-COV-2, influenza A virus and influenza B virus could be detected simultaneously in the same reaction tube, and the amplification correlation coefficient of each detected target gene was high, the linear relationship was good, and there was no obvious interference between each other.

    Case 2: After 10-fold gradient dilution of SARS-COV-2, influenza A virus and influenza B virus samples, Bioer BSC86 was used for purification.

    Figure 2 ORF1ab gene of SARS-COV-2

    Figure 3 N gene of SARS-COV-2

    Figure 4. Influenza A virus

    Figure 5 Influenza B virus

    The results showed that the amplification correlation coefficients were all above 0.995 with a good linear relationship.




    Manual download
  • SARS-CoV-2 Nucleic Acid Detection Kit MORE
    Product Details:

    This product selects the ORF1ab and N gene regions of novel coronavirus SARS-CoV-2, and designs two sets of specific primers and fluorescent probes that cover two sites of the gene at the same time.  In order to control the entire extraction and detection process, human β-Globin gene was act as a non-competitive internal control during the extraction and detection process. The RT-PCR reaction solution is pre-mixed, which is convenient, efficient, and easy to operate. It utilizes high-efficiency HotStart Taq DNA Polymerase and has strong stability. The kit could be used for auxiliary diagnosis and epidemiological surveillance of novel coronavirus SARS-CoV-2 infection.

    Product Features:

    1.Stable and high-efficiency: Utilize HotStart Taq DNA Polymerase, the stability is strong.

    2. High specificity and precision: Primers and probes are designed for specific fragments of two gene regions, which confirm each other to    make the results more accurate. Human β-Globin gene as the internal control is included into the reagent to verify the validity of the experiment.

    3. Convenience: The reaction solution is mixed in advance to complete RT-PCR in one step, saving time and effort.

    4. Multiple Real-time RT-PCR detection: Each channel does not interfere with each other, and the amplification curve is S-shaped.

    Experimental data:
    1. Homogeneity experiment: Take two sets of SARS-CoV-2 virus nucleic acid samples (106copies/mL and 104copies/mL) in two groups with different concentrations, respectively. And utilize BIOER's SARS-CoV-2 nucleic acid detection kit to do the detection, the test results are as follows.

    Nucleic acid Con.(106copies/ml)

    FAM

    Ct Value

    HEX

    Ct Value

    Cy5

    Ct Value

    Nucleic acid Con.(104copies/ml)

    FAM

    Ct Value

    HEX

    Ct Value

    Cy5

    Ct Value

    1

    23.94

    24.63

    27.89

    1

    31.13

    32.13

    28.08

    2

    23.92

    24.54

    27.83

    2

    31.31

    32.1

    27.89

    3

    23.88

    24.50

    27.89

    3

    31.20

    32.26

    28.10

    4

    23.95

    24.79

    27.96

    4

    31.23

    32.14

    27.69

    5

    24.06

    24.75

    27.94

    5

    31.22

    32.12

    28.14

    6

    23.94

    24.56

    27.98

    6

    31.21

    32.06

    27.88

    7

    23.88

    24.55

    27.88

    7

    31.07

    31.87

    27.77

    8

    23.97

    24.49

    27.91

    8

    31.08

    32.03

    28.06

    9

    24.03

    24.57

    27.83

    9

    31.16

    32.07

    27.86

    10

    23.99

    24.59

    27.94

    10

    31.12

    32.10

    27.95

    11

    23.93

    24.45

    27.80

    11

    31.12

    32.13

    27.88

    12

    24.02

    24.51

    27.88

    12

    31.34

    32.28

    28.00

    13

    24.01

    24.71

    27.86

    13

    31.38

    32.25

    28.05

    14

    23.90

    24.59

    27.85

    14

    31.15

    32.08

    28.19

    15

    23.97

    24.73

    27.90

    15

    31.22

    32.04

    27.84

    The results showed that the three channels of the SARS-CoV-2 nucleic acid detection kit had good uniformity.

     

    2.Stability experiment: 96 samples of SARS-CoV-2 nucleic acid were randomly selected and tested by BIOER's SARS-CoV-2 nucleic acid detection kit to verify the stability of the internal control of Cy5 channel; SARS-CoV-2 nucleic acid samples at the concentration of 5×102copies/mL were tested by the SARS-CoV-2 nucleic acid detection kit to verify the stability of the lower limit. The results are as follows:

    Manual download
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