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Magabio Method

  • MagaBio plus Fecal pathogens DNA/RNA Purification Kit MORE
    Product Details:

    The kit uses a unique lysis formula and the grinding tube contains unique grinding beads, which can fully grind fecal samples. Using magnetic bead technology, DNA/RNA can be isolated from pathogens at the same time. The purified high quality nucleic acids purified by this kit can be directly used for downstream molecular biological detection, such as PCR/RT-PCR, Mutant, SNP, NGS

    Applicable instrument:especially suitable for Bioer NPA-32+, NPA-32P, NPA-96 nucleic acid purification instruments


     

    Product Features:

    1. Fast and efficient purification of pathogen nucleic acid

    2. High safety, does not contain toxic reagent

    3. Stable and reliable, it can extract unknown pathogen samples

    4. High extraction sensitivity

    5. Convenient transportation and storage

  • MagaBio pathogens DNA/RNA Purification Kit MORE
    Product Details:

    High quality DNA/RNA was purified from pathogens in a variety of samples, including plasma, alveolar lavage fluid, sputum, saliva, animal tissue samples, etc. Equipped with company sputum liquefaction at the same time, the alveolar lavage fluid, sputum, viscous samples have good liquefaction effect such as saliva, sputum liquefaction agent from reserve cells, viruses and bacteria integrity, improve the efficiency of sputum liquefaction and eliminating DNA polymer composites and other aspects of sputum liquefaction, can be used for DNA/RNA virus in the sputum and purification process of pretreatment, greatly improved the extraction efficiency of this kind of sample.

    Product Features:

    1. Strong applicability: It is suitable for the nucleic acid extraction of pathogens causing respiratory diseases such as novel coronavirus, mycoplasma, chlamydia and bacteria, etc. For special samples, such as viscous alveolar lavage fluid, sputum, saliva, etc., there is a unique liquefaction agent, which greatly improves the extraction effect of such samples.

    2. A wide range of samples: it can easily extract nucleic acids of pathogens such as sputum, saliva, alveolar lavage fluid, (nasopharyngeal) swabs, culture cells and other body fluid samples (such as whole blood, plasma, serum, ascites, cerebrospinal fluid, oral fluid, etc.);

    3. High efficiency: it can combine 95% nucleic acid in the solution, and only takes 60 minutes to complete whole process

    4. High sensitivity: DNA virus can reach 10 IU/mL, RNA virus can reach 50 IU/mL

    5. Simple operation: the unique pre-treatment method of the bath, combined with the pre-packaged kit, adding samples and PK can be operated on the machine to ensure the repeatability of many experiments;

    6. High safety: the reagent is non-toxic and harmless

    Experimental data:

    The Magabio pathogens DNA/RNA purification kit was used to extract the diluted HBV virus samples from human serum (10-105 IU/mL) and detect its performance by fluorescence quantitative amplification. The results were as follows:

    A:Fluorescence amplification curve

     

    B:Standard curve result

    The results of nucleic acid fluorescence amplification were extracted from HBV samples

    Concentration of the sample IU/mL 

    CT Valuerepetition 

    105

    18.8/18.8

    104

    21.92/22.06

    103

    25.38/25.52

    102

    28.5/28.55

    10

    31.87/31.6

    Negative control

    -


    Manual download
  • MagaBio plus General Genomic DNA Purification Kit II MORE
    Product Details:

    This kit is suitable for extracting genomic DNA from blood, saliva, dried blood spots, buccal swabs, animal tissues and other samples.The kit uses a set of standardized operating procedures: sample processing, magnetic bead adsorption, washing and elution. GenePure automatic nucleic acid extraction and purification instruments can be used to extract 32-96 samples at a time.

     

    Single Sample Size

    Whole blood, saliva and other body fluid samples≤250μL,

    Animal tissue≤50mg, Cultured cells≤1×;108

    Dried blood spots: 3×3mm 3~8 pieces, 6×6mm 1~3 pieces 

    DNA Yield

    Whole blood, saliva and other body fluid samples≥2μg/200μL;

    Animal tissue : 2~15μg/30mg

    Cultured cells : 2~8μg/5×105 

    DNA Purity

    OD260/OD280:1.7~2.1

    Operation Time

    45-50min

    Supporting Tools

    Magnetic separation rack (optional)

    Supporting Instrument

    Automatic nucleic acid extraction and  purification instrument

    Product Features:

    Wide range of application coverage, it can be applied to most samples such as blood, body fluids, tissues, cells, buccal swabs, dried blood spots, etc. It is extremely versatile.

    Simple and Fast, suitable for various automated system

    Safe and non-toxic, no organic reagents such as phenol imitation

    Stable and precise performance, High yield 

    Experimental data:

    Application Case 1

     The concentration and purity of the genomic DNA of various samples purified by MagaBio plus General Genomic DNA Purification Kit II are very high, and the concentration detected by Qubit is very good, indicating that the extraction performance of the kit is stable and efficient, and the genomic DNA with high concentration and high purity can be obtained. The diagram below:

    Gel electrophoresis of DNA extracted from different samples

    Sample Type

    Nano

    ng/µL 

    A260/A280

    A260/A230

    Qubit

    ng/µL 

    Nano/Qubit

    Dried blood spots

    3mm*8 pieces

    27.3

    1.94

    1.43

    20.90

    1.31

    Dried blood spots

    3mm*8 pieces

    24.2

    1.86

    1.43

    17.80

    1.36

    30mg Kidney tissue

    262.1

    1.88

    2.09

    263.00

    1.00

    30mg Kidney tissue

    250.5

    1.88

    2.62

    252.00

    0.99

    250μL Whole Blood

    226.8

    1.89

    2.01

    193.4

    1.17

    250μL Whole Blood

    197.3

    1.92

    2.12

    172.4

    1.14

    250μL saliva

    123.1

    1.73

    1.99

    92.00

    1.34

    250μL saliva

    241.7

    1.81

    2.21

    204.00

    1.18

    Application Case 2 

    Using MagaBio plus General Genomic DNA Purification Kit II to extract genomic DNA from human buccal swabs, the electropherogram and concentration detection results are as follows: (Comparison between BIOER and TG)

    Electrophoresis of DNA extracted from human buccal swab

    Sample Name

    Nanong/ul 

    A260/A280

    1-TG

    39.6

    1.5

    2-TG

    43.5

    1.6

    3-TG

    23

    1.8

    1-BIOER

    58.4

    1.78

    2-BIOER

    45.4

    1.81

    3-BIOER

    35.7

    1.85


     

     

     

    The results show that: BIOER products are better than TG in terms of concentration and purity of oral swab samples.

    Application Case 3

    Using MagaBio plus General Genomic DNA Purification Kit II to extract genomic DNA from a human whole blood sample (200ul), the electropherogram and concentration detection results are as follows: (Comparison between BIOER and M ) 

    Electrophoresis of DNA extracted from whole blood sample

    Sample Name

    Nanong/ul

    A260/A280

    Sample Name

    Nanong/ul

    A260/A280

    Bioer-1

    74.40

    1.98

    Company M-1

    61.4

    1.9

    Bioer-2

    109.60

    1.93

    Company M-2

    60.9

    1.91

    Bioer-3

    52.00

    1.97

    Company M-3

    37.7

    1.79

    Bioer-4

    80.60

    1.94

    Company M-4

    59.9

    1.9

    Bioer-5

    67.20

    1.92

    Company M-5

    45.7

    1.93

    Bioer-6

    57.40

    1.98

    Company M-6

    41.9

    1.91

    Bioer-7

    130.60

    1.77

    Company M-7

    33.5

    1.9

    Bioer-8

    77.20

    1.95

    Company M-8

    57.3

    1.84

    From the above results, it can be seen that BOIER's extraction concentration of human whole blood samples is better than that of Company M

    Manual download
  • MagaBio plus Whole Blood Genomic DNA Purification Kit II MORE
    Product Details:

    This kit can be used to extract DNA from various anticoagulants (anticoagulants including citrate, heparin, Na2EDTA) human whole blood, serum, white blood cells and other special samples.

    The kit uses a set of standardized operating procedures: sample processing, magnetic bead adsorption, washing and elution. GenePure automatic nucleic acid extraction and purification instruments can be used to extract 32-96 samples at a time.

     

    Single Sample Size

    ≤240μL whole blood

    DNA Yield

    ≥2μg/200μL

    DNA Purity

    OD260/OD280:1.7~2.1

    Operation Time

    45-50min

    Supporting Tools

    Magnetic separation rack (optional)

    Supporting Instrument

    Automatic nucleic acid extraction and purification instrument

     

    Product Features:

    1.Simple and Fast,suitable for various automated system

    2.Safe and non-toxic, no organic reagents such as phenol imitation

    3.Stable and precise performance, High yield 

    Experimental data:

    DNA extraction is performed with 240μL human blood sample. After extraction, the concentration detection and electrophoresis diagram are as follows:


    Sample Name

    Nanong/ul 

    A260/A280

    Fresh Blood-1

    60

    1.81

    Fresh Blood-2

    63

    1.86

    Fresh Blood-3

    60.2

    1.86

    Fresh Blood-4

    63.7

    1.85

    Fresh Blood-5

    68.4

    1.86

    Fresh Blood-6

    72.9

    1.86

    Frozen Blood

    60.1

    1.76

    Frozen Blood

    66.2

    1.79

    Non-Fresh Blood -1

    33

    1.85

    Non-Fresh Blood -2

    36.9

    1.84

    Non-Fresh Blood -3

    39.2

    1.8

    Non-Fresh Blood -4

    34.3

    1.81

    Non-Fresh Blood -5

    37.6

    1.82

    Non-Fresh Blood -6

    35.7

    1.8

    Manual download
  • MagaBio plus Virus DNA Purification Kit II MORE
    Product Details:

    This kit can quickly and efficiently isolate viral DNA from samples such as serum, plasma, feces and body fluids. Virus nucleic acid in whole blood, serum, plasma and body fluid is released under the action of the Lysis buffer. The released nucleic acid is specifically bound to the magnetic beads in the binding buffer. The magnetic beads with the nucleic acid are captured by the magnetic material, and the contaminants are removed by the washing buffer. Finally, the DNA is eluted from the magnetic beads under the action of the Elution buffer. The purified DNA can be widely used in PCR, sequencing, mutant analysis and other common molecular biology experiments.

     

    Single Sample Size

    300μL

    DNA Purity

    OD260/OD280: 1.7-2.1

    Sensitivity

    5IU/mL

    Supporting Tools

    Magnetic separation rack (optional)

    Applicable instrument:

     GenePure series automatic nucleic acid extraction and purification instrument

    Product Features:

    1. Simple, Fast, High-throughput

    2. Safe and non-toxic, no organic reagents such as phenol imitation

    3. Optimization for small samples

    4. Suitable for various automated system

    Experimental data:

    DNA extracted from HBV-positive serum samples (10-107 IU/ml) was used as a template to perform HBV Real-time PCR experiments. The results are as follows:

    Sample

    Ct

    1(E4

    25.11

    2(E3

    28.78

    3(E2

    31.88

    4(10)

    35.76

     

    Manual download
  • MagaBio Soil and Feces Genomic DNA Purification Kit MORE
    Product Details:

    This kit is suitable for extracting genomic DNA from soil and feces. Add an appropriate amount of soil or feces into the grinding tube. Under the action of SP Buffer and Lysis S Buffer, shake the grinding tube vigorously, and the genomic DNA is released. Most impurities are removed with the method of centrifugation. The unique DA Buffer can effectively precipitate impurities, such as humic acid, protein, and polysaccharides. The released DNA is specifically bound to the magnetic beads, and the magnetic beads that bind the DNA are captured by the magnetic material. The contaminants are removed by multiple washing processes. Finally, the DNA is washed from the magnetic beads under the action of the elution buffer.

    Applicable instrument:

     GenePure series automatic nucleic acid extraction and purification instrument

     

    Single Sample Size

    300μL

    DNA Purity

    OD260/OD280: 1.7-2.1

    Supporting Tools

    Magnetic separation rack (optional)

    Product Features:

    1. Simple, Fast, High-throughput

    2. Special formula can effectively remove impurities such as humic acid and fulvic acid

    3. Safe and non-toxic, no organic reagents such as phenol imitation

    4. Suitable for various automated system

    Experimental data:

    Use 0.5g soil as sample to extract genomic DNA, the elution volume is 100μL, The results of agarose gel electrophoresis and Nano Drop detection are as follow:

    Marker:DL2000 1-2:Soil samples

     

    Sample ID

    Nucleic Acid

    260/280

    260/230

    0.5g sludge-1

    98.60

    1.73

    1.02

    0.5g sludge-2

    106.40

    1.77

    1.08

     

     

    Manual download
  • MagaBio Plasma Circulating DNA Purification Kit MORE
    Product Details:

    This kit is suitable for extracting circulating cell-free DNA (cfDNA) from plasma, serum and other cell-free interstitial fluid. The purified cfDNA has high yield and good quality. This kit can remove protein, lipid and other inhibitory impurities to the maximum. The extracted nucleic acid can be directly used in gene sequencing (non-invasive prenatal genetic testing, tumor detection), library construction and other downstream experiments.

     

    Single Sample Size

    300μL

    DNA Purity

    OD260/OD280: 1.7-2.1

    DNA Yield

    500ng-1μg

    Supporting Tools

    Magnetic separation rack (optional)

    Applicable instrument:

    GenePure series automatic nucleic acid extraction and purification instrument

    Product Features:

    1.Simple, Fast, High-throughput

    2.Stable and precise performance, High yield

    3. Safe and non-toxic, no organic reagents such as phenol imitation

    4. Suitable for various automated system

    Experimental data:

    The circulating cell-free DNA was extracted from 300μL plasma.

    Electrophoresis result:

                                     ;                   M:Marker DL2000 1-6:Circulating cell-free DNA

    Sample ID

    OD260

    OD280

    260/280

    Concentration(μg/μL)

    1

    1.161

    0.455

    2.5

    58.1

    2

    1.175

    0.466

    2.5

    58.7

    3

    1.12

    0.443

    2.5

    56

    4

    1.177

    0.491

    2.4

    58.8

    5

    1.193

    0.491

    2.4

    59.7

    6

    1.168

    0.478

    2.4

    58.4

     

    Manual download
  • MagaBio Plus virus RNA Extraction kit II MORE
    Product Details:

    This kit is suitable for the extraction of viral RNA from serum, plasma, tissue, swab (after sampling), stool and
    body fluid samples. With the COVID-19 test for the most commonly used nasopharyngeal swab samples, this product can finally complete the viral RNA extraction process in 8 minutes after continuous product performance optimization.


    Principle:

    Features Specifications
    Method Magnetic beads
    Sample Serum, plasma, tissue, swab (after sampling), stool and body fluid samples
    Sample Volume 10μL -300μL
    Elution Volume 70μL
    Processing Time 8 minutes
    Yield High purity viral RNA
    Storage Conditions 2-8°C
    Shelf life 12 months

     

     
    Product Features:

    1.Rapid extraction: The viral RNA extraction process was completed in 8 minutes.
    2.Easy to use: No need to add protease K, convenient and fast.
    3.High sensitivity: The sensitivity can reach 50 IU/mL after the quality control test calibrated by the national.
    4.Good stability: Meet the high-quality requirements of Bioer Technology.

    Experimental data:

    This kit was used to extract RNA from coronavirus samples, and real-time PCR was used to detect the sensitivity and stability of the kit

    ResultBioer MagaBio Plus virus RNA extraction kit II product showed superior sensitivity and stability under the premise of completing the viral RNA extraction in 8 minutes.


    Manual download
  • MagaBio Plus virus DNA/ RNA extraction kit III MORE
    Product Details:

    MagaBio Plus virus DNA/RNA extraction kit III compared to the previous two generations, this product uses a unique lysate system to rapidly lyse the virus, combined with a new magnetic bead technology for the purification of nucleic acids.

    The sample types cover whole blood, tissues, plasma, serum, body fluids, animal and plant tissues, feces, swabs,
    and alveolar lavage fluid, etc., which are more common and do not require protease K.

    96 samples can be easily extracted in only 15 minutes. The purified nucleic acid can be used for detection of NGS, qPCR, etc.

    Principle:

    Features Specifications
    Method Magnetic beads
    Sample Serum, Stool, Plasma, Whole blood, Swabs, Saliva, Body fluid Tissue etc
    Sample Volume 10μL -300μL
    Elution Volume 70μL
    Processing Time 15 minutes
    Yield High purity viral DNA or viral RNA
    Storage Conditions 2-8°C
    Shelf life 12 months
    Product Features:

    1.Strong versatility: Suitable for samples of tissues, feces, whole blood, serum, plasma and body fluids.
    2.Easy to use: No need to add protease K
    3.Rapid extraction: 96 samples can be extracted in 15 minutes
    4.High sensitivity: High sensitivity is achieved through the quality control product test calibrated by the national standard.

    Experimental data:

    Determination of the extraction sensitivity of MagaBio Plus virus DNA/RNA Purification kit III (BSC86), using HCV RNA virus with a national
    standard concentration of double dilution of negative serum. The extracted products were detected by HCV RNA detection reagent with high sensitivity, and the results were shown in Figure 1 and Figure 2:

    ResultThe sensitivity of MagaBio Plus virus DNA/RNA Purification kit III to extract HCV RNA virus can reach 50IU/mL, indicating that the product has high sensitivity


     

    Manual download
  • MagaBio plus Virus DNA/RNA Purification Kit II MORE
    Product Details:

    The MagaBio plus Virus DNA/RNA Purification Kit II is designed for the rapid, efficient and automatic isolation and purification of high quality virus nucleic acid from a variety of samples on the automatic nucleic acid extraction system. It takes about only 35 minutes for the total procedure. The obtained virus nucleic acid can be used directly for a broad range of downstream applications, such as qPCR, NGS, etc

    Principle:

    Features Specifications
    Method Magnetic beads
    Sample Serum, Plasma, Whole blood, Swabs, Saliva, Body fluid Tissue, Feces, BALF, etc
    Sample Volume 20-1000 μL
    Elution Volume 80μL
    Processing Time 35 minutes
    Yield High purity viral DNA or viral RNA
    Storage Conditions 2-8°C
    Shelf life 12 months
       
       
    Product Features:

    1.Rapid and Reliable: Fast procedures and easy to use

    2.High Sensitivity: DNA: 5 IU/mL, RNA: 50 IU/mL

    3.High Efficiency: ≥ 95% of the nucleic acid can be combined.

    4.Versatile: Nucleic acid of various virus, e.g. SARS-CoV-2, Hepatitis, etc. in different samples, such as Serum, Plasma, Whole blood, Swabs, Saliva, Body fluid, Tissue, Feces, BALF, etc. for a broad range of down stream applications.

    5.Safe: Toxic free

    Experimental data:

    Case 1 To Extract DNA from HBV

    Gradient dilute the HBV positive plasma sample, extract the HBV DNA of the original and diluted plasma samples by using MagaBio plus Virus DNA/RNA Purification Kit II, followed with the quantitative detection of HBV DNA by HBV Quantitative PCR test kit in real-time PCR system.

    The results show that the extraction kit has very good performance in extracting HBV DNA from plasma sample and the extraction concentration can be 10 IU/mL .

    Case 2 To Extract RNA from HCV

    Gradient dilute the HCV positive serum sample, extract the HCV RNA of the original and diluted serum samples by using MagaBio plus Virus DNA/RNA Purification Kit II, followed with the quantitative detection of HCV RNA by HCV Quantitative RT-PCR test kit in real-time PCR system.

    The results show that the extraction kit has very good performance in extracting HCV RNA from serum sample and the extraction concentration can be 50 IU/mL


    Manual download
  • MagaBio plus Plant Genomic DNA Purification Kit MORE
    Product Details:

    The MagaBio Plant Genomic DNA Purification Kit provides a very simple, fast and effective technique to isolate high quality DNA. By one simple protocol, purified DNA in high yield can be isolated from various sources including maize, tea, rice, onion and petunia.
    MagaBio sample processing is based on proprietary magnetizable particles-MagaBio. The pure DNA can be applied extensively in PCR, sequencing, Southern hybridization, mutant analysis, SNP and the others.
    A standardized process (sample preparation, beads binding, washing and elution) is established to purify 32 samples once by using our
    Nucleic Acid Purification System.

     

     

    Item Description
    Sample Volume 25-100mg plant tissue
    DNA Performance 2-10μg
    DNA Purity OD
    260/OD280: 1.7~2.0
    Operation time 35min(32 samples by NPA-32+ instrument)
    45min(2-12 samples by manual)
    Accessories Magnetic rack(Optional)
    Support Equipment NPA-32+ GenePure Plus Nucleic Acid Purification System




    Product Features:

    1.Suitable for most of plant sample

    2.Simple, effective and safe process, no centrifugation

    3.No organic solvent and other toxic reagent

    4.Excellent performance for sample with plenty of polysaccharide and polyphenol

    5.Suitable for automatic platform


    Experimental data:

    1.Plant DNA purification by NPA-32+ instrument, Electrophoresis results

    M λ DNA/Hind marker
    1
    2345 25mg Rice leaf

    2.Plant DNA purification by NPA-32+ instrument, Electrophoresis results

    M λ DNA/Hind marker
    1
    23 30mg Soybean
    4
    56 30mg Plant roots tissue


    Manual download
  • MagaBio Blood Spots Genomic DNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC34S1E

    BSC34S1

    BSC34M1

    Components

    32 Tests

    50 Tests

    100 Tests

    TES Buffer

    11.2ml

    17.5 ml

    35 ml

    Protease K (PK)

    320 μl

    500 μl

    1000 μl

    Lysis Buffer

     

     

     

    96 well pre- packed plates

    2 pieces

    25ml

    50ml

    WB1 Buffer

    7 m l×2

    (add 10.5 ml absolute ethanol before use)

    14 m l×2

    (add 21 ml absolute ethanol before use)

    Wash Buffer

    6.3ml×2

    (add 25.2 ml absolute ethanol before use)

    12.6 ml×2

    (add 50.4 ml absolute ethanol before use)

    Elution Buffer

    10 ml

    20 ml

    MagaBio Reagent

    0.75 ml

    1.5 ml

    Handbook V1.0

    1

    1

    1

    Introduction

        The kit provides a very simple, fast and cost effective technique to isolate high quality DNA. Using one simple protocol, high yield of purified DNA can be isolated from Blood Spots. MagaBio sample  processing is based on proprietary magnetizable particles--MagaBio. The pure DNA can be applied extensively in PCR, sequencing, Southern hybridization, mutant analysis, SNP and the others.

        According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage

     

    • 1.The kit can be transported at room temperature.
    • 2.The kit should be stored at 2~8℃.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.

     

    Advantage

    MagaBio Magnetic technical have great advantages:

     

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No organic solvent
    • 5.No high salt solution, no inhibitor
    • 6.No spin column, no centrifuge

     

    Apparatus and materials to be prepared by the user

     

    • 1.Magnetic Rack or Bioer NPA-32P purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC34S1&For BSC34M1)

     

    Important Notes

    • 1. This kit is for research use only.
    • 2. Before you begin, you should read this user’s manual carefully.
    • 3. The use of nuclease-free lab ware (e.g. pipettes, pipette tips, reactions vials) as well as.
    • 4. Wearing gloves when performing the assay.
    • 5. To avoid cross-contamination of samples and reagents use fresh aerosol-preventive pipette tips for all pipetting steps.
    • 6. After the experiment, please disinfect the workbench with 75% ethanol or 10% hypochlorous acid, and sterilize the workplace by UV lamp.
  • MagaBio Blood Spots Genomic DNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC34S1C

    BSC34S1

    BSC34M1

    Components

    32 Tests

    50 Tests

    100 Tests

    TES Buffer

    11ml

    17.5 ml

    35 ml

    Protease K (PK)

    320 μl

    500 μl

    1000 μl

    Lysis Buffer

     

     

     

    96 well pre- packed plates

    2 pieces

    25ml

    50ml

     WB1 Buffer

    7 m l×2

    (add 10.5 ml absolute ethanol before use)

    14 m l×2

    (add 21 ml absolute ethanol before use)

     Wash Buffer

    6.3ml×2

    (add 25.2 ml absolute ethanol before use)

    12.6 ml×2

    (add 50.4 ml absolute ethanol before use)

    Elution Buffer

    10 ml

    20 ml

    MagaBio Reagent

    0.75 ml

    1.5 ml

    Handbook V1.0

    1

    1

    1

    Introduction

        The kit provides a very simple, fast and cost effective technique to isolate high quality DNA. Using one simple protocol, high yield of purified DNA can be isolated from Blood Spots. MagaBio sample  processing is based on proprietary magnetizable particles--MagaBio. The pure DNA can be applied extensively in PCR, sequencing, Southern hybridization, mutant analysis, SNP and the others.

        According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage
    • 1.The kit can be transported at room temperature. 
    • 2.The kit should be stored at 2~8℃.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.

    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No organic solvent
    • 5.No high salt solution, no inhibitor
    • 6.No spin column, no centrifuge

    Apparatus and materials to be prepared by the user

    • 1.Magnetic Rack or Bioer NPA-32+ purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC34S1&For BSC34M1)

    Important Notes

    • 1. This kit is for research use only.
    • 2. Before you begin, you should read this user’s manual carefully.
    • 3. The use of nuclease-free lab ware (e.g. pipettes, pipette tips, reactions vials) as well as.
    • 4. Wearing gloves when performing the assay.
    • 5. To avoid cross-contamination of samples and reagents use fresh aerosol-preventive pipette tips for all pipetting steps.
    • 6. After the experiment, please disinfect the workbench with 75% ethanol or 10% hypochlorous acid, and sterilize the workplace by UV lamp.
  • MagaBio plasmid DNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC41T1E

    Components

    16Tests

    RNase A Solution

    16 μl

    Resuspension Buffer

    4 ml

    Lysis Buffer

    4 ml

    Neutralization Buffer

    2.4 ml

    Lysis Buffer

     

     

    96 well pre-packed plate 1 piece

    WB1 Buffer

    Wash Buffer

    Elution Buffer

    MagaBio Reagent

    Handbook

    1

    Introduction

        The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. Plasmid DNA can be purified from 1–5ml of overnight cultures of E. coli. MagaBio sample processing is based on proprietary magnetic particles—MagaBio. The DNA isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-time PCR and other downstream experiments.

    Product Features:

    Storage

    • 1.The kit can be transported at room temperature.
    • 2.All reagents are stored at 2-8°C.
    • 3.All reagents, when stored properly, are stable for 18 months from the time of delivery.

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000 × g            * Vortex mixer

    Important notes

    • 1. The RNase A solution should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
    • 2. If the Lysis Buffer precipitated, it should be dissolved by warming to 37°C.
    • 3. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
    • 4. The kit can extract high-quality plasmid DNA from 1-5ml E.coli overnight cultured.
  • MagaBio plus Bacterium DNA Fast Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC45S1E

    Components

    32 Tests

    TET Buffer

    6.4 ml

    Lysozyme

    128 mg

    PK Solution

    640 μl

    Lysis Buffer

     

     

     

    96 well pre-packed plate 2 pieces

    WB1 Buffer

    Wash Buffer

    Elution Buffer

    MagaBio Reagent

    Handbook V1.0

    1

    Introduction

        The kit provides a very simple, fast and effective technique to isolate high quality bacteria DNA. Using one simple protocol, high yield of purified DNA can be isolated from various sources bacteria including gram-positive bacteria and gram-negative bacteria. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio Reagent. The pure DNA can be applied extensively in PCR, sequencing, southern hybridization, mutant analysis, SNP and the others. According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:

    Storage

     

    • 1.The kit can be transported at room temperature.
    • 2.The kit should be stored at 2~8℃.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.

     

     Advantage

    MagaBio Magnetic technical have great advantages:

     

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No organic solvent
    • 5.No high salt solution, no inhibitor

     

    Apparatus and materials to be prepared by the user

     

    • 1.Magnetic Rack or Bioer NPA-32P purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC09S1B and BSC09M1B)

     

     

    Important Notes

    • 1. This kit is for research use only.
    • 2. Before you begin, you should read this user’s manual carefully.
    • 3. The use of nuclease-free lab ware (e.g. pipettes, pipette tips, reactions vials) as well as.
    • 4. Wearing gloves when performing the assay.
    • 5. After the experiment,please disinfect the workbench with 75% ethanol or 10% hypochlorous acid, and sterilize the workplace by UV lamp.
  • MagaBio plus Bacterium Genomic DNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC09S1G

    BSC09S1B

    Components

    32 Tests

    50 Tests

    RB Buffer

    4 ml

    5.5 ml

    Lysozyme

    40 mg

    55 mg

    PK Solution

    320 μl

    0.5ml

    RNase A Solution

    64 μl

    100 μl

    Lysis Buffer

    3.2 ml

    5 ml

     Binding Buffer

     

     

    96 well pre-packed plate

    2 pieces

    12 ml

    (add18ml absolute ethanol before use)

     Wash Buffer

    24ml

    (add 36ml absolute ethanol before use)

     Elution Buffer

     10 ml

     MagaBio Reagent

     0.5 ml

    Handbook V1.0

    1

    1

    Introduction

        The kit provides a very simple, fast and effective technique to isolate high quality bacteria DNA. Using one simple protocol, high yield of purified DNA can be isolated from various sources bacteria including gram-positive bacteria and gram-negative bacteria. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio Reagent. The pure DNA can be applied extensively in PCR, sequencing, southern hybridization, mutant analysis, SNP and the others. According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage
    • 1.The kit can be transported at room temperature. 
    • 2.The kit should be stored at 2~8℃.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.
    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No organic solvent
    • 5.No high salt solution, no inhibitor

    Apparatus and materials to be prepared by the user

    • 1.Magnetic Rack or Bioer NPA-32P purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC09S1B and BSC09M1B)
    • Important Notes
    • 1. This kit is for research use only.
    • 2. Before you begin, you should read this user’s manual carefully.
    • 3. The use of nuclease-free lab ware (e.g. pipettes, pipette tips, reactions vials) as well as.
    • 4. Wearing gloves when performing the assay.
    • 5. After the experiment,please disinfect the workbench with 75% ethanol or 10% hypochlorous acid, and sterilize the workplace by UV lamp.
  • MagaBio plus Bacterium Genomic DNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC09S1C

    BSC09S1B

    Components

    32 Tests

    50 Tests

    4%NaOH

    40ml×3

    60 ml×3

    RB Buffer

    4 ml

    5.5 ml

    Lysozyme

    40 mg

    55 mg

    PK Solution

    320 μl

    0.5ml

    RNase A Solution

    64 μl

    100 μl

    Lysis Buffer

    3.2 ml

    5 ml

     Binding Buffer

     

     

    96 well pre-packed plate

    2 pieces

    12 ml

    (add18ml absolute ethanol before use)

     Wash Buffer

    24ml

    (add 36ml absolute ethanol before use)

     

    Elution Buffer

     

    10 ml

     

    MagaBio Reagent

     

    0.5 ml

    Handbook V1.0

    1

    1

    Introduction

        The kit provides a very simple, fast and effective technique to isolate high quality bacteria DNA. Using one simple protocol, high yield of purified DNA can be isolated from various sources bacteria including gram-positive bacteria and gram-negative bacteria. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio Reagent. The pure DNA can be applied extensively in PCR, sequencing, southern hybridization, mutant analysis, SNP and the others. According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage

    • 1.The kit can be transported at room temperature.
    • 2.The kit should be stored at 2~8℃.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.
    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No organic solvent
    • 5.No high salt solution, no inhibitor
    Apparatus and materials to be prepared by the user

    • 1.Magnetic Rack or Bioer NPA-32+ purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC09S1B and BSC09M1B)

    Important Notes

    • 1. This kit is for research use only.
    • 2. Before you begin, you should read this user’s manual carefully.
    • 3. The use of nuclease-free lab ware (e.g. pipettes, pipette tips, reactions vials) as well as.
    • 4. Wearing gloves when performing the assay.
    • 5. After the experiment,please disinfect the workbench with 75% ethanol or 10% hypochlorous acid, and sterilize the workplace by UV lamp.
  • MagaBio plus Total RNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    Sample

    Components

    10 tests

    ML Buffer

    4 ml

    Binding Buffer

    6 ml

    DNase Stop Buffer

    2.8 ml

    (add 4.2 ml absolute  ethanol before use)

    Wash Buffer

    3.9 ml

    (add 9.1 ml absolute ethanol before use)

    RElution Buffer

    2 ml

    MagaBio Reagent

    0.1 ml

    Handbook V1.0

    1

    Introduction

        The kit provides a very simple, fast and cost effective technique to isolate high quality RNA. Using one simple protocol, high yield of purified RNA can be isolated from various sources including blood, culture cells, leukocytes, tissue and plant. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio. The pure RNA can be applied extensively in RT-PCR, Northern hybridization, mutant analysis, RNAi and the others.

        According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage

    • 1.The kit can be transported at room temperature.
    • 2.All reagents are stored at room temperature.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.
    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1. Mini sample, high purification
    • 2. Simple and streamline separation procedure, used for auto-platform
    • 3. First elution can acquire 85% or more
    • 4. No high salt solution. no inhibitor
    • 5. No spin column

    Apparatus and materials to be prepared by the user

    • 1.Magnetic Rack or Bioer NPA-32+ purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol
    Important Notes
    • 1. Add the alcohol (as the volume marked on bottle label) to the DNase Stop Buffer and Wash Buffer, and then mix them well.
    • 2.  MagaBio Reagent should be mixed well (particles are uniformly suspended) every time.
    • 3. MagaBio plus total RNA Purification Kit has been optimized for use with fresh whole blood and leukocytes, frozen blood samples cannot be used.
    • 4. Proper microbiological, asceptic technique should always be followed when working with RNA. All plasticware and glassware should be free of RNase contamination. Keep the tubes closed during the procedure, work quickly, and keep the purified RNA on ice until used.
    • 5.  If the room temperature is too low ,we can put the ML Buffer at 45℃ warm bath for preheating.
  • MagaBio plus Total RNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC53T1E

    Components

    16Tests

    ML Buffer

    6.4 ml

    Binding Buffer

     

     

     96 well pre-packed plate 1 piece

    DNase Stop Buffer

    Wash Buffer

    RElution Buffer

    MagaBio Reagent

    Handbook V1.0

     

    Introduction

        The kit provides a very simple, fast and cost effective technique to isolate high quality RNA. Using one simple protocol, high yield of purified RNA can be isolated from various sources including blood, culture cells, leukocytes, tissue and plant. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio. The pure RNA can be applied extensively in RT-PCR, Northern hybridization, mutant analysis, RNAi and the others.

        According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage

    • 1.The kit can be transported at room temperature.
    • 2.All reagents are stored at room temperature.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.
    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1. Mini sample, high purification
    • 2. Simple and streamline separation procedure, used for auto-platform
    • 3. First elution can acquire 85% or more
    • 4. No high salt solution. no inhibitor
    • 5. No spin column
    Apparatus and materials to be prepared by the user
    • 1.Bioer NPA-32P purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    Important Notes
    • 1. Add the alcohol (as the volume marked on bottle label) to the DNase Stop Buffer and Wash Buffer, and then mix them well.
    • 2. MagaBio Reagent should be mixed well (particles are uniformly suspended) every time.
    • 3. MagaBio plus total RNA Purification Kit has been optimized for use with fresh whole blood and leukocytes, frozen blood samples cannot be used.
    • 4. Proper microbiological, asceptic technique should always be followed when working with RNA. All plastic ware and glassware should be free of RNase contamination. Keep the tubes closed during the procedure, work quickly, and keep the purified RNA on ice until used.
    • 5. If the room temperature is too low ,we can put the ML Buffer at 45℃ warm bath for preheating.
  • MagaBio plus Total RNA Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC53S1C

    BSC53S1B

    BSC53M1B

    Components

    32 tests

    50tests

    100tests

    ML Buffer

    12.8 ml

    20 ml

    40 ml

    Binding Buffer

     

     

     

    96 well pre-packed plate

    2 pieces

    30 ml

    60 ml

    DNase Stop Buffer

    12.6 ml

    (add 18.9 ml absolute ethanol before use)

    25.2 ml

    (add 37.8 ml absolute ethanol before use)

    Wash Buffer

    18.9 ml

    (add 44.1 ml absolute ethanol before use)

    18.9 ml×2

    (add 44.1 ml absolute ethanol before use)

    RElution Buffer

    10 ml

    20 ml

    MagaBio Reagent

    0.5 ml

    1 ml

    Handbook V1.0

     

    1

    1

    Introduction

        The kit provides a very simple, fast and cost effective technique to isolate high quality RNA. Using one simple protocol, high yield of purified RNA can be isolated from various sources including blood, culture cells, leukocytes, tissue and plant. MagaBio sample processing is based on proprietary magnetizable particles--MagaBio. The pure RNA can be applied extensively in RT-PCR, Northern hybridization, mutant analysis, RNAi and the others.

        According to the special interaction, use MagaBio nucleic acid separation system with a general protocol---sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

    Product Features:
    Storage

    • 1.The kit can be transported at room temperature.
    • 2.All reagents are stored at room temperature.
    • 3.All reagents, when stored properly, are stable for 12 months from the time of delivery.
    Advantage

    MagaBio Magnetic technical have great advantages:

    • 1.Mini sample, high purification
    • 2.Simple and streamline separation procedure, used for auto-platform
    • 3.First elution can acquire 85% or more
    • 4.No high salt solution. no inhibitor
    • 5.No spin column

    Apparatus and materials to be prepared by the user

    • 1.Magnetic Rack or Bioer NPA-32+ purification instrument
    • 2.Water bath or Dry bath
    • 3.Vortex mixer
    • 4.Absolute alcohol (For BSC53S1B and BSC53M1B)

    Important Notes

    • 1.Add the alcohol (as the volume marked on bottle label) to the DNase Stop Buffer and Wash Buffer, and then mix them well.
    • 2.MagaBio Reagent should be mixed well (particles are uniformly suspended) every time.
    • 3.MagaBio plus total RNA Purification Kit has been optimized for use with fresh whole blood and leukocytes, frozen blood samples cannot be used.
    • 4.Proper microbiological, aseptic technique should always be followed when working with RNA. All plastic ware and glassware should be free of RNase contamination. Keep the tubes closed during the procedure, work quickly, and keep the purified RNA on ice until used.
    • 5.If the room temperature is too low ,we can put the ML Buffer at 45℃ warm bath for preheating.
12
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