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Reagent Center

Spin Column Method

  • Biospin Plasmid DNA Extraction Kit MORE
    Product Details:

    Kit Components 

    Cat#

     

    BSC01M1

     

    BSC01L1

    Components

    100Tests

    250Tests

    Resuspension Buffer

    25ml

    62.5ml

    Lysis Buffer

    25ml

    62.5ml

    Neutralization Buffer

    35ml

    87.5ml

    Wash Buffer

    30 ml

    (add 45ml absolute ethanol before use) ×2

    75 ml

    (add 112.5ml absolute ethanol before use) ×2

    Elution Buffer

    20ml

    50ml

    RNase solution

    1

    1

    Spin columns

    100

    250

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. Plasmid DNA can be purified from 1–5ml of overnight cultures of E. coli. The DNA isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-time PCR and other downstream experiments.

    Product Features:

    Storage

    1.The kit should be stored at room temperature (15~25℃), but the RNase solution should be stored at 2~8℃.The kit can be stored for up to 18 months by this method. After addition of RNase solution, Resuspension Buffer should be stored 2~8℃.

    2.The kit can be transported at room temperature.

    Technical Information

     

    Method

     

    Work time

     

    Column volume

     

    Column yield

     

    Elution recovery

     

    Plasmid DNA length

     

    Culture volume

    Spin column

    25 min for

    24 samples

    750µl

    20µg DNA

    ≥99%

    ≤10kB

    1~5ml high-copy  plasmid

    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml micro centrifuge tubes   ;            * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000 × g           ; * Absolute ethanol       ;   * Vortex mixer

    Important notes

    1. The RNase solution should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
    2. Add ethanol (as the volume is marked on bottle label) to Wash Buffer and mix well.
    3. If the Lysis Buffer and Neutralization Buffer precipitated, it should be Redissolved by warming to 37°C. Please not vortex Lysis Buffer acutely.
    4. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
    5. The kit can extract high-quality plasmid DNA from 1-5ml E.coli overnight cultured.
    6. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
    Experimental data:

    DNA Analysis

    • Absorbance anlysis,

    Get some plasmid DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:  2.0≥OD260-320/ OD280-320≥1.8

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis,0.8~1% Agarose gel 

     

    Example 2:Enzymatic reactions analysis

    Manual download
  • Biospin Plasma Circulating DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC30S1

    BSC30M1

    Components

    50Tests

    100Tests

    Solution  Ⅰ

    20ml

    40ml

    Solution  Ⅱ

    10ml

    20ml

    PK solution

    500ul  (Store at 2~8℃)

    1000ul  (Store at 2~8℃)

    LysisB Buffer

    10ml

    20ml

    WB1 Buffer

    12ml

    (add 17ml ethanol before use)

    24ml

    (add 34ml ethanol before use)

    Wash Buffer

    18ml

    (add 42ml ethanol before use)

    36ml

    (add 84ml ethanol before use)

    Elution Buffer

    10 ml

    20 ml

    Spin column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a very simple, fast and effective technique for the isolation of pure plasma DNA. DNA in the sample is released using PK solution and LysisB Buffer. The released DNA is bound exclusively and specifically to the Biospin membrane in presence of LysisB Buffer and ethanol under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed with  twice washing procedures. The DNA is then eluted from the membrane with the elution Buffer. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. The pure DNA can be applied extensively in PCR/Real-time PCR, and so on.

    Product Features:

    Storage and transportation 

    • The PK solution must be stored at 2~8℃, other components in the kit may be deposited at room temperature.
    •  All components, when stored properly, can keep stable for 18months.
    •  The kit can be transported at room temperature.

    Apparatus and materials to be prepared by the user

    *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 12,000×g       ;            * Absolute ethanol (>99%)

    *  Vortex mixer                                                                 * Warm bath or metal bath

    Important note

    Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

  • Biospin PCR Purification Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC03S1

    BSC03M1

    Components

    50Tests

    100Tests

    Binding Buffer

    10ml

    20ml

    Wash Buffer

    15ml

    30ml

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit provides a simple, rapid and effective method for purification of DNA fragments from PCR or enzymatic reaction. DNA fragments ranging from 60bp to 10kb can be purified. The yield of DNA with size lower than 100bp is 23~95%, while the yield of DNA with size from 0.1kb to 10kb is 90~97%. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, PCR/real-time PCR and so on.

    Product Features:

    Storage and Transportation

    • The kit should be stored dry at room temperature (15~25℃), The kit can be stored for up to 18 months if all components are kept in the manner above.
    • The kit can be transported at room temperature.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5 microcentrifuge tubes          * 10µl/100µ;l/1000µl tips

    *  Microcentrifuge capable of 14,000g     * Vortex mixer ;    * Absolute ethanol

    Important notes

    1. Add ethanol (as the volume be marked on bottle label) to Wash Buffer and mix well
    2. Close the lid after using the Binding Buffer as soon as possible.
    3. The suitable volume is 50ul for Elution Buffer; user can adjust its volume if necessary.
    Experimental data:

    Analysis DNA

    • Absorbance anlysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.8

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:

    According to the absorbance and agarose gel analysis,the impurity had been discarded.

    U: unpurified      P: purified               M: Marker

    Example 2:

    Example3:Elution Volume versus DNA Yield

     

    Manual download
  • Biospin Omni Plant Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC13S1B

    Components

    50Tests

    LP Buffer

    22.5 ml

    LP plus Buffer

    22.5 ml

    DA Buffer

    7.5 ml

    P Binding Buffer

    14ml

    G Binding Buffer

    25ml

    Wash Buffer

    25.2ml

    Elution Buffer

    10ml

    Spin column

    50

    Shredder Spin Column

    50

    Handbook

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high– molecular-weight genomic DNA from plant tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and  hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

     

    Product Features:

    Storage

    • The kit should be stored at 15-25℃.
    •  All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml microcentrifuge tubes           ;  * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g ;    ;         * Absolute ethanol            * Vortex mixer

    Important notes

    1. Please add 37.8ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    2. Please add 28ml absolute ethanol to P Binding Buffer and mix thoroughly before the  first use.
    3. LP Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    Absorbance anlysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    expressions:concentration(μg/ml)=50×OD260×dilution fact

    target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:Rice leaves

    Example2: The extraction of plant tissues rich in polysaccharides and polyphenols

                      

    Manual download
  • Biospin miRNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC64S1

    BSC64M1

    Components

    50Tests

    100Tests

    BIOZOL Reagent

    35ml

    70ml

    Wash Buffer

    12ml

    (add 48ml ethanol before use)

    24ml

    (add 96ml ethanol before use)

    Reslution Buffer

    15ml

    30ml

    Spin columns

    50

    100

    Handbook

    1copy

    1copy

    Introduction

        The kit is a ready-to-use reagent for the isolation of total RNA,including miRNA and other small RNA molecules, from cultured cells and various animal and human tissues. Add BIOZOL Reagent to the processed sample, after addition of chloroform, RNA will be isolated from DNA and Protein, and adding alcohol will bind RNA all RNA molecules from 18 nucleotides (nt) upwards to spin column. Then RNA  can be easily isolated through several washing and eluting steps.

        The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library, microarray analysis etc.

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 12 months when the BIOZOL Reagent should be stored at 2-8  ℃,others at room temperature.
    • The kit can be transported at room temperature.
    Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml microcentrifuge tubes                ;    ;  * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm                  * Absolute alcohol

    *  Vortex mixer    ;          ;      ;                         ;   ;      * Chloroform

    Important note

    Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.

    Experimental data:

    Analysis RNA

    • Absorbance analysis

    Get some RNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320.

    Expressions:concentration(μg/ml)=40×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Real-Time PCR Analysis (Rat rno-miR-21)

    Manual download
  • Biospin Marine Animal Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC27S1

    BSC27M1

    Components

    50T

    100T

    FL Buffer

    30ml

    60ml

    PK Solution

    0.5ml

    1ml

    WS Buffer

    5ml

    10ml

    Binding Buffer

    35ml

    70ml

    PW Buffer

    12ml

    (add 18ml ethanol before use)

    24ml

    (add 36ml ethanol before use)

    Wash Buffer

    26ml

    (add 39ml ethanol before use)

    52ml

    (add 78ml ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from marine animal tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA without proteins and other contaminants. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The PK solution is to be stored at 2-8℃, others at 15-25℃.
    •  All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    * Sterile 2.0ml microcentrifuge tubes                * 10µl/100µl/1000µl tips

    * Microcentrifuge capable of 14,000g                * Absolute ethanol

    Important notes

    1. Please add3.6ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
    2. Please add 8.4ml absolute ethanol to Wash Binding Buffer and mix thoroughly before the first use.
    3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target:2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.8~1% Agarose gel

    Example 1:Fishtail

    Example 2:Shell meat

    Manual download
  • Biospin Insect Genomic DNA Extraction Kit MORE
    Product Details:
    Kit Components

    Cat#

    BSC26S1

    BSC26M1

    Components

    50Tests

    100Tests

    FL Buffer

    30.0ml

    60.0ml

    PK solution

    1ml

    2ml

    Binding Buffer

    35.0ml

    70.0ml

    PW Buffer

    11ml(add 16.5ml ethanol before use)

    22ml(add 33ml ethanol before use)

    Wash Buffer

    25ml(add 37.5ml ethanol before use)

    50ml(add 75ml ethanol before use)

    Elution Buffer

    10.0ml

    20.0ml

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from insect, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps. It can effectively remove all kinds of pigment, chitin, polysaccharide and other impurities. It can also extract multiple samples once.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The PK Solution is to be stored at 2-8℃, others at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5/2.0ml microcentrifuge tubes          * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g               * Absolute ethanol    ;     * Chloroform

    Important notes

    1. Please add 33ml absolute ethanol to PW Buffer and mix thoroughly before the first use.

    2. Please add 75ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.

    3. FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

     

  • Biospin Fungus Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components 

    Cat#

    BSC14S1

    BSC14M1

    Components

    50Tests

    100Tests

    LE Buffer

    20 ml

    40 ml

    DA Buffer

    7.5 ml

    15 ml

    E Binding Buffer

    14ml

    (add 28 ml absolute ethanol before use)

    28ml

    (add 56 ml absolute ethanol before use)

    G Binding Buffer

    25ml

    50ml

    Wash Buffer

    25.2ml

    (add 37.8ml absolute ethanol before use)

    50.4ml

    (add 75.6ml absolute ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.

        The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:

    Storage

    • The kit should be stored at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml micro centrifuge tubes              ; * 10µl/100µl/1000µ;l tips

    *  Microcentrifuge capable of 14,000g   ;           * Absolute ethanol     * Vortex mixer

    Important notes

    1. Please add absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    2. Please add absolute ethanol to E Binding Buffer and mix thoroughly before the first use.
    3. LE Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
    4. Prepare Sorbitol buffer: 18.217 g Sorbitol and 3.7224 g EDTA-2Na, dissolved in 100 ml of pure water.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260   ,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact Target:        ;     ;   2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:

    Manual download
  • Biospin FFPE Tissue RNA Extraction Kit MORE
    Product Details:

    Kit Components 

     

    Cat#

     

     

    BSC66S1

     

     

    BSC66M1

    Components

    50Tests

    100Tests

    Deparaffinization Solution

    50ml

    100ml

    Lysis II Buffer

    10ml

    20ml

    Binding Buffer

    10ml

    20ml

    SW Buffer

    26ml

    52ml

    Wash Buffer II

    8ml

    (Add 32 ml ethanol before use)

    16ml

    (Add64 ml ethanol before use)

    RElution Buffer

    10ml

    20ml

    PK Solution

    500µl (Stored at 2-8℃)

    1ml (Stored at 2-8℃)

    Spin Column

    50

    100

    Handbook

    1 copy

    1 copy

    Cat#

    BSA35S2B (Stored at -20)

    BSA35M2B (Stored at -20)

    DNase I Buffer

    1.3 mlх2

    1.3 mlх4

    DNase I

    100µl

    200µl

    Introduction

        This kit is used to extract high- purity RNA from FFPE tissue sections, with non-toxic deparaffinization solution, high-performance Lysis  II Buffer to release RNA from FFPE  efficiently. The high efficient binding of RNA to our spin matrix while proteins and other impurities are removed by   wash buffer. Nucleic acids are easily eluted with sterile RNase free water or RElution Buffer. The purified RNA is ready for downstream applications such as Real-Time PCR,Sec

    Product Features:

    Storage and transportation

    • The kit has demonstrated stability of 12 months when the PK Solution should be stored at 2-8℃,DNase I should be stored at -20℃,others at room temperature.
    • The kit can be transported at room temperature, but PK Solution and DNase I need cold chain.

    Apparatus and materials to be prepared by the user

    *  1.5ml microcentrifuge tubes              ;   ;    ;   * 10µl/100µl/1000µl tips

    *  Centrifuge capable of ≥14,000g                 * Ethanol(≥95%)

    *  Heating block or water bath

    Important note

    1)Wash Buffer II: Add the ethanol as the volume marked on bottle label and mix well.

    2)Lysis II Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

    3)PK Solution should be stored at 2°;C-8℃.

    4)DNase I should be stored at -20°C.

    5)Carry out all centrifugations at room temperature.

    Experimental data:

    Analysis RNA

    Real-Time PCR compare

    Manual download
  • Biospin Endo-free Plasmid DNA Mini Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC01S2D

    BSC01M2D

    Components

    50Tests

    100Tests

     

    Resuspension Buffer

     

    15 ml

     

    30 ml

     

    Lysis Buffer

     

    15 ml

     

    30 ml

     

    N3 Buffer

     

    4 ml

     

    8 ml

     

    KB Buffer

     

    30 ml

     

    60 ml

     

    RET Buffer

     

    30 ml

     

    60 ml

     

    DNA Wash Buffer

    15 ml

    (Add 60 ml ethanol before use)

    30 ml

    (Add 120 ml ethanol before use)

     

    Endofree Elution Buffer

     

    10 ml

     

    20 ml

     

    RNase A (20mg/ml)

     

    75 μl (1.5 mg )

     

    150 μl (1.5 mg )

     

    Mini Spin Columns

     

    50

     

    50

    Handbook

    1copy

    1copy

    Introduction

        Plasmid isolated with traditional protocol normally contains high level of endotoxins (Lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. This kit uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level   to 0.1 EU (Endotoxin) per μg of plasmid DNA. The endofree plasmid midiprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA.

        This kit is designed for fast and efficient purification of plasmid DNA from 3 to 5 mL of E. coli culture. The mini column has a DNA binding capacity of 50 μg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.

    Product Features:

    Storage and transportation

    •  Resuspension Buffer should be stored at 2-8°C once RNase A is added. All other materials can be stored at room temperature (22-25°C).
    • The Guaranteed shelf life is 12 months from the date of purchase.

    Apparatus and materials to be prepared by the user

    *  100% ethanol                 ;                                    * High speed centrifuge

    *  1.5/2ml high speed centrifuge tubes             * 10µ;l/100µl/1000µl tips

    Important note

    • RNase A: Add the RNase A solution to Resuspension Buffer and mix well before use. Store at 2-8°C.  
    • Lysis Buffer: Precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use. Keep the cap tightly closed for Lysis Buffer after use.
    • N3 Buffer: Precipitates below 10  ℃,warm up at 37  ℃ to dissolve  the precipitates before use.
    • DNA Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.
  • Biospin Cell Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC05S1

    BSC05M1

    Components

    50Tests

    100Tests

    PK solution

    0.5 ml

    1 ml

    LC Buffer

    5 ml

    10 ml

    GA Buffer

    10 ml

    20 ml

    G Binding Buffer

     

    40ml

     

    80ml

     

    Wash Buffer

    25.2 ml

    (add37.8ml absolute ethanol before use)

    50.4 ml

    (add 75.6ml absolute ethanol before use)

    Elution Buffer

    20 ml

    40 ml

    Spin column

    100

    200

    Handbook

    1copy

    1copy

     

    Introduction

        The kit provides a very simple, fast and economic way for the isolating of pure high– molecular-weight genomic DNA from all kinds of cultured cells, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, Biospin Cell Genomic DNA Extraction kit is applied for the purification of at least 100 cells.

        The pure DNA can be applied extensively in PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

    Product Features:
    Storage

     

    • The PK solution is to be stored at 2-8℃,others at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.
    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml microcentrifuge tubes               * 10µl/100µ;l/1000µl tips

    *  Microcentrifuge capable of 14,000g       ;    ;   * Absolute ethanol     * Vortex mixer

    Important notes

    • Please add absolute ethanol to wash buffer and mix thoroughly before use.
    •  In order to optimize the effective result, the appropriate number of cultured cell is between 100 and 5×106, which is suspended in 100μl PBS buffer or 0.9%NaCl.
    •  LC Buffers may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:
    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260   ,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact Target:    2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.8~1% Agarose gel

    Example 1:

    Example 2:comparation of different companies

    Manual download
  • Biospin Bacteria Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC12S1

    BSC12M1

    Components

    50Tests

    100Tests

    Proteinase K

    0.5ml

    1ml

     

    EL Buffer

     

    5ml

     

    10ml

     

    RS Buffer

     

    5 ml

     

    10 ml

    GA Buffer

    10 ml

    20 ml

     

    BA Buffer

    10ml

    (add 10.5ml ethanol before use)

    20ml

    (add 21ml ethanol before use)

    G Binding Buffer

    25ml

    50ml

     

    Wash Buffer

    21ml

    (add 31.5ml ethanol before use)

    42ml

    (add 63ml ethanol before use)

    Elution Buffer

    10ml

    20ml

    Spin column

    50

    100

    Handbook

    1 copy

    1 copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolating of pure high–molecular-weight genomic DNA from all kinds of bacterial, including Gram-Negative Bacterial and Gram-Positive Bacterial, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, the kit can get at most 30ug genomic DNA from 5×109 Bacterial cells.The pure DNA can be applied extensively in PCR/Real time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

     

    Product Features:

    Storage

    • The Proteinase K is to be stored at 2-8℃,others at 15-25℃.
    • All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 1.5ml micro centrifuge tubes   ;          * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000 × g          * Absolute ethanol           * Vortex mixer

    Important notes

    • Gram negative bacteria(optional step):Please add158mg Lysozyme to EL Buffer, mix thoroughly for 30seconds or so until the solution is clear, stored at 2-8℃.

         Gram positive bacteria: Please add 158mg Lysozyme to EL Buffer, mix thoroughly for 30seconds or so until the solution is clear, stored at 2-8℃.

    • Please add 10.5ml absolute ethanol to BA Buffer and mix thoroughly before use.
    • Please add 31.5ml absolute ethanol to Wash Buffer and mix thoroughly before use.
    • order to optimize the effective result, the appropriate number of bacterial cells is at most 5×109, which OD600 is between 1.0~2.0.
    • RS Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 56°C until the precipitate has fully dissolved.

     

    Experimental data:
    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260, OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact Target: 2.0≥OD260-320/ OD280-320≥1.7

    Notice:  1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis 0.81Agarose gel

    Example 1:Different amount

    Example 2:G+: gram-negative, G-: gram-positive

     

    Manual download
12
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