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Aquatic Pathogen Series

  • Aquatic Pathogen Series Reagents MORE
    Product Details:

    In this series of products, primers and TaqMan probes are designed with virus specific gene fragments to realize rapid qualitative detection of viral nucleic acids in aquatic and economic animals such as shrimp and fish. It can be used for virus tracking detection in the breeding process, and also for environmental monitoring, so as to avoid the spread of virus and reduce economic losses. The series also has multi-channel reagents that simultaneously detect two diseases in the same tube.

     

     

    Main component

    Storage temperature

    Amplification reagents

    PCR reaction reagents

    -15~-25℃

    Primers probe

    Control

    Negative control

    Positive control

     

    Product list

    Cat#

    Product Name

    Package

    Note

    BSN01S1

    White Spot Syndrome Virus Real Time PCR Detection Kit

    16T

    -20℃Storage

    BSN01M1

    32T

    BSN02S1

    Infectious hypodermal and haematopoietic necrosis virus Real Time PCR Detection Kit

    16T

    BSN02M1

    32T

    BSN03S1

    Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit

    16T

    BSN03M1

    32T

    BSN04S1

    Vibrio Harveyi Real Time PCR Detection Kit

    16T

    BSN04M1

    32T

    BSN05S1

    EMS & EHP Dual Chanel Real Time PCR Detection kit

    16T

    BSN05M1

    32T

    BSN06S1

    Bacteria &; Vibrio Dual Chanel Real Time PCR Detection Kit

    16T

    BSN06M1

    32T

    BSN07S1

    Taura Syndrome Virus Real Time PCR Detection Kit

    16T

    BSN07M1

    32T

    BSN08S1

    Yellow head virus Real Time PCR Detection Kit

    16T

    BSN08M1

    32T

    BSN09S1

    Enterocytozoon hepatopenaei Real Time PCR Detection Kit

    16T

    BSN09M1

    32T

    BSN10S1

    Acute Hepatopancreatic Necrosis Disease Real Time PCR Detection Kit

    16T

    BSN10M1

    32T

     


    Product Features:

    1. It is suitable for the Bioer fluorescence quantitative PCR detection system and the same kind of real-time quantitative detection system

    2. High sensitivity: detection limit up to 100 copies/mL

    3. High specificity: The design of specific primers and probes can safely avoid cross reactions and the resulting false positives

    4. Easy to operate, short time consuming: the reagent adopts premixed form, less components, easy to operate, only need about 1 hour to complete whole experiment.

    5. DUTP-UNG system is used to effectively prevent contamination of PCR products

    Experimental data:

    Experiment 1

    White Spot Syndrome Virus Real Time PCR Detection Kit

    (1) Linear tests with concentrations of 102 to 107 copies/mL of DNA showed good results, R2=0.9985

    DNA(copies/mL)

    Ct

    Ct value

    106

    19.18

    19.2

    19.22

    105

    22.64

    22.63

    22.62

    104

    26.31

    26.37

    26.43

    103

    29.82

    29.83

    29.84

    102

    34.1

    34.11

    34.12

     

     

     

     

    (2) The test was repeated using lower concentrations of DNA

     

    The results showed that the detection rate of low concentration DNA was 100%, and the reproducibility was good


    Experiment 2

     Dual nucleic acid detection reagent linear test of acute hepatopancreas necrosis (EMS) and hepatoenterocytic worm (EHP) (DNA concentration 102 ~ 107copies/mL)

    DNA(copies/mL)

    EHP(FAM)

    EMS(HEX)

    106

    16.93

    16.42

    105

    20.3

    19.95

    104

    24.12

    23.41

    103

    27.73

    27.22

    102

    31.62

    31.48

     




































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